RESUMO
SU-8 is an epoxy-based, biocompatible thermosetting polymer, which has been utilized mainly to fabricate biomedical devices and scaffolds. In this study, thin, single-layered, freestanding tuneable porous SU-8 membranes were microfabricated and surface hydrophilized for efficient bioseparation. Unlike the previous thicker membranes of 200-300 µm, these thin SU-8 membranes of 50-60 µm thickness and pores with 6-10 µm diameter were fabricated and tested for blood-plasma separation, without any additional support structure. The method is based on making a patterned SU-8 layer by electrospin coating and UV lithography on a sacrificial polyethylene terephthalate (PET) sheet attached to a silicon wafer. Poor adhesion between PET and SU-8 aid in the convenient release of the thin porous membranes with uniform pore formation. The single-layered self-supporting membranes were strong, safe, sterilizable, reusable, and suitable for plasma separation and postfermentation broth enrichment.
Assuntos
Plasma , Polímeros , Porosidade , SilícioRESUMO
Juvenile shrimps (Penaeus monodon) weighing about 15-20 gms were procured from the grow-out ponds and reared under laboratory conditions. They were challenged with Vibrio parahaemolyticus MTCC 451 and their LD50 value was evaluated. The ascorbic acid and the histamine activity of both the control and experimental groups were assessed. The results showed a marked decline in both the parameters at all intervals. This reveals the pathogenesis of the inoculated bacterial strain, which affects the defense mechanism by reducing the glutathione content by the decreased level of ascorbic acid and letharginess by the decreased histamine content of the experimental prawns.
